HLA-B27 Binding Assay - http://www.its.caltech.edu/~bjorker/Protocols/B27_binding_assay.pdf Bjorkman Lab. HLA-B27 Binding Assay 1) As of today the B27 protein concentration = 9 mg/mL 2) Dilute the protein 2-fold with 1xPBS: 75 μl B27 (9 mg/mL) 75 μl 1xPBS 2 μl 0.1M DTT 3) Incubate on ice for 5 minutes. 4) Meanwhile, prepare the spin columns (Biospin 6 and Biospin 30 from Biorad; they are stored in the cold room). Biospin -6 column equilibration: - [Read more]